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Expanding the Scope of Laccase-Mediator Systems

Authors

  • Dr. Paul Könst,

    1. Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL Delft (The Netherlands), Fax: (+31) 15-278-1415
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    • These authors contributed equally to this work.

  • Dr. Selin Kara,

    1. Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL Delft (The Netherlands), Fax: (+31) 15-278-1415
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    • These authors contributed equally to this work.

  • Svenja Kochius,

    1. Biochemical Engineering, DECHEMA Research Institute, Theodor-Heuss-Allee 25, 60486 Frankfurt am Main (Germany)
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  • Dr. Dirk Holtmann,

    1. Biochemical Engineering, DECHEMA Research Institute, Theodor-Heuss-Allee 25, 60486 Frankfurt am Main (Germany)
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  • Prof. Dr. Isabel W. C. E. Arends,

    1. Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL Delft (The Netherlands), Fax: (+31) 15-278-1415
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  • Dr. Roland Ludwig,

    1. Department of Food Science and Technology, BOKU – University of Natural Resources and Life Sciences, Muthgasse 11, 1190 Wien (Austria)
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  • Dr. Frank Hollmann

    Corresponding author
    1. Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL Delft (The Netherlands), Fax: (+31) 15-278-1415
    • Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL Delft (The Netherlands), Fax: (+31) 15-278-1415
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Abstract

The laccase-mediator system (LMS) for the regeneration of oxidised nicotinamide co-factors was revisited to broaden the mediator scope. Among the 18 mediators screened, acetosyringone, syringaldehyde and caffeic acid excelled with respect to activity and stability under process conditions. The LMS based on the laccase from Myceliophthora thermophila and acetosyringone was further investigated and applied to promote the nicotinamide adenine dinucleotide (NAD+)-dependent oxidation of glucose as well as the oxidative lactonisation of 1,4-butanediol to the corresponding γ-butyrolactone.

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