Biocatalytic Asymmetric Synthesis of Unnatural Amino Acids through the Cascade Transfer of Amino Groups from Primary Amines onto Keto Acids

Authors

  • Eul-Soo Park,

    1. Department of Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, 120-749 Seoul (South Korea), Fax: (+82) 2-362-7265
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  • Joo-Young Dong,

    1. Department of Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, 120-749 Seoul (South Korea), Fax: (+82) 2-362-7265
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  • Prof. Dr. Jong-Shik Shin

    Corresponding author
    1. Department of Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, 120-749 Seoul (South Korea), Fax: (+82) 2-362-7265
    • Department of Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, 120-749 Seoul (South Korea), Fax: (+82) 2-362-7265

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Abstract

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Flee to the hills: An unfavorable equilibrium in the amino group transfer between amino acids and keto acids catalyzed by α-transaminases was successfully overcome by coupling with a ω-transaminase reaction as an equilibrium shifter, leading to efficient asymmetric synthesis of diverse unnatural amino acids, including L-tert-leucine and D-phenylglycine.

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