An Enzymatic Toolbox for Cascade Reactions: A Showcase for an In Vivo Redox Sequence in Asymmetric Synthesis

Authors

  • Nikolin Oberleitner,

    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Christin Peters,

    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Jan Muschiol,

    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    Search for more papers by this author
  • Dr. Maria Kadow,

    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    Search for more papers by this author
  • Stefan Saß,

    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    Search for more papers by this author
  • Thomas Bayer,

    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    Search for more papers by this author
  • Patricia Schaaf,

    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    Search for more papers by this author
  • Dr. Naseem Iqbal,

    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    Search for more papers by this author
  • Dr. Florian Rudroff,

    Corresponding author
    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    • Florian Rudroff, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Marko D. Mihovilovic, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Uwe T. Bornscheuer, Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367

    Search for more papers by this author
  • Prof. Dr. Marko D. Mihovilovic,

    Corresponding author
    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    • Florian Rudroff, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Marko D. Mihovilovic, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Uwe T. Bornscheuer, Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367

    Search for more papers by this author
  • Prof. Dr. Uwe T. Bornscheuer

    Corresponding author
    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    • Florian Rudroff, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Marko D. Mihovilovic, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Uwe T. Bornscheuer, Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367

    Search for more papers by this author

Abstract

original image

Joined forces: Alcohol dehydrogenase, enoate reductase, and Baeyer–Villiger monooxygenase are combined in a cascade reaction by coexpression in E. coli to have a recombinant whole-cell biocatalyst. Such an artificial metabolic “mini”-pathway provides access to functionalized chiral compounds in high yields and optical purities as exemplified for kinetic resolutions, desymmetrizations, and regiodivergent biotransformations.

Ancillary