Get access

An Enzymatic Toolbox for Cascade Reactions: A Showcase for an In Vivo Redox Sequence in Asymmetric Synthesis

Authors

  • Nikolin Oberleitner,

    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Christin Peters,

    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Jan Muschiol,

    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    Search for more papers by this author
  • Dr. Maria Kadow,

    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    Search for more papers by this author
  • Stefan Saß,

    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    Search for more papers by this author
  • Thomas Bayer,

    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    Search for more papers by this author
  • Patricia Schaaf,

    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    Search for more papers by this author
  • Dr. Naseem Iqbal,

    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    Search for more papers by this author
  • Dr. Florian Rudroff,

    Corresponding author
    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    • Florian Rudroff, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Marko D. Mihovilovic, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Uwe T. Bornscheuer, Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367

    Search for more papers by this author
  • Prof. Dr. Marko D. Mihovilovic,

    Corresponding author
    1. Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)
    • Florian Rudroff, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Marko D. Mihovilovic, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Uwe T. Bornscheuer, Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367

    Search for more papers by this author
  • Prof. Dr. Uwe T. Bornscheuer

    Corresponding author
    1. Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367
    • Florian Rudroff, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Marko D. Mihovilovic, Institute of Applied Chemistry, Technical University Vienna, Getreidemarkt 9/163-OC, 1060 Vienna (Austria)

      Uwe T. Bornscheuer, Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald (Germany), Fax: (+49) 3834-86-794367

    Search for more papers by this author

Abstract

original image

Joined forces: Alcohol dehydrogenase, enoate reductase, and Baeyer–Villiger monooxygenase are combined in a cascade reaction by coexpression in E. coli to have a recombinant whole-cell biocatalyst. Such an artificial metabolic “mini”-pathway provides access to functionalized chiral compounds in high yields and optical purities as exemplified for kinetic resolutions, desymmetrizations, and regiodivergent biotransformations.

Get access to the full text of this article

Ancillary