Cover Picture: Tailoring the S-Selectivity of 2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate Synthase (MenD) from Escherichia coli (ChemCatChem 12/2013)



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Stereoselective traffic engineering The cover picture shows how engineered S-selective EcMenD variants catalyze the carboligation of α-ketoglutarate and benzaldehyde derivatives with excellent enantioselectivities of up to 99 % ee S, in contrast to the wild-type enzyme, which produces R-enantiomers. In their Full Paper on p. 3587 ff., M. Pohl et al. describe how they engineer S-selective EcMenD variants by optimizing steric properties and stabilization of the acceptor substrate in the S-pocket. This method, inspired by the recently developed S-pocket concept, involves opening the S-pocket with simultaneous destabilization of the R-pathway to enhance the S-selectivity of thiamine diphosphate-dependent enzymes.

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