Lipase Congeners Designed by Genetic Code Engineering (pages 213–221)
Michael G. Hoesl, Carlos G. Acevedo-Rocha, Sebastian Nehring, Dr. Marina Royter, Dr. Christina Wolschner, Dr. Birgit Wiltschi, Prof. Dr. Nediljko Budisa and Prof. Dr. Garabed Antranikian
Article first published online: 19 OCT 2010 | DOI: 10.1002/cctc.201000253
Gene genius: Congeners of lipase from Thermoanaerobacter thermohydrosulfuricus containing globally substituted methionine, proline, phenylalanine, and tyrosine with related synthetic analogues (see examples in figure) have special features not found in nature. These features include changes in substrate accessibility and tolerance, as well as optimal temperature, pH, and enzymatic activity profiles.