Microscopy Methods used to Elucidate Protein Adsorption and Transport Behavior on Novel Chromatographic Media
Top left: polarization anisotropy map for fluorescently labeled lysozyme in solution and adsorbed on SP Sepharose XL particles. Top right (courtesy of Dr. Harun Koku): SEM of Sepharose FF (left half) and Sepharose XL (right half), both with protein adsorbed. Bottom right: photobleaching of fluorescently labeled lysozyme adsorbed on SP Sepharose FF. Bottom left: confocal microscopy of simultaneous loading of fluorescently labeled lysozyme (green) and lactoferrin (red) on SP Sepharose FF.
B. D. Bowes, S. J. Traylor, S. M. Timmick, K. J. Czymmek, A. M. Lenhoff*
Insights into Protein Sorption and Desorption on Dextran-Modified Ion-Exchange Media
Chem. Ing. Technol.2012, 35 (1), 91–101