Conference Review

iSPOT: a web tool for the analysis and recognition of protein domain specificity

A presentation for the ESF workshop ‘Proteomics: Focus on Protein Interactions’

  1. Barbara Brannetti,
  2. Andreas Zanzoni,
  3. Luisa Montecchi-Palazzi,
  4. Gianni Cesareni,
  5. Manuela Helmer-Citterich*

Article first published online: 10 OCT 2001

DOI: 10.1002/cfg.104

Issue

Comparative and Functional Genomics

Comparative and Functional Genomics

Volume 2, Issue 5, pages 314–318, October 2001

Additional Information

How to Cite

Brannetti, B., Zanzoni, A., Montecchi-Palazzi, L., Cesareni, G. and Helmer-Citterich, M. (2001), iSPOT: a web tool for the analysis and recognition of protein domain specificity. Comparative and Functional Genomics, 2: 314–318. doi: 10.1002/cfg.104

Author Information

  1. Department of Biology, University of Rome Tor Vergata, Rome, Italy

*Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy.

Publication History

  1. Issue published online: 10 OCT 2001
  2. Article first published online: 10 OCT 2001
  3. Manuscript Accepted: 27 JUL 2001
  4. Manuscript Received: 28 JUN 2001

Funded by

  • EEC. Grant Number: QLR1-CT-2000-00127
  • The CNR target project in Biotechnology

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Keywords:

  • prediction of specificity;
  • molecular recognition;
  • protein domains;
  • computational methods;
  • web tools;
  • homology modelling

Abstract

Methods that aim at predicting interaction partners are very likely to play an important role in the interpretation of genomic information. iSPOT (iSpecificity Prediction Of Target) is a web tool (accessible at http://cbm.bio.uniroma2.it/iSPOT) developed for the prediction of protein-protein interaction mediated by families of peptide recognition modules. iSPOT accesses a database of position specific residue-residue interaction frequencies for members of the SH3 and PDZ protein domain families. The software utilises this database to provide a score for any potential domain peptide interaction.

 iSPOT: 1. evaluates the likelihood of the interaction between any of the peptides contained in an input protein and a list of domains of the two different families; 2. searches in the SWISS-PROT database for potential partners of a query domain; and 3. has access to a repository of all the domain/target peptide interaction data. Copyright © 2001 John Wiley & Sons, Ltd.

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