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Solid-Phase Synthesis of Lipidated Peptides

Authors

  • Maria Lumbierres Dr.,

    1. Max-Planck-Institut für molekulare Physiologie, Abteilung Chemische Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Universität Dortmund, Fachbereich 3, Chemische Biologie, Germany, Fax: (+49) 231-133-2499
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  • Jose M. Palomo Dr.,

    1. Max-Planck-Institut für molekulare Physiologie, Abteilung Chemische Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Universität Dortmund, Fachbereich 3, Chemische Biologie, Germany, Fax: (+49) 231-133-2499
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  • Goran Kragol Dr.,

    1. Max-Planck-Institut für molekulare Physiologie, Abteilung Chemische Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Universität Dortmund, Fachbereich 3, Chemische Biologie, Germany, Fax: (+49) 231-133-2499
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  • Sonja Roehrs,

    1. Max-Planck-Institut für molekulare Physiologie, Abteilung Strukturelle Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
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  • Oliver Müller Dr.,

    1. Max-Planck-Institut für molekulare Physiologie, Abteilung Strukturelle Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
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  • Herbert Waldmann Prof. Dr.

    1. Max-Planck-Institut für molekulare Physiologie, Abteilung Chemische Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany
    2. Universität Dortmund, Fachbereich 3, Chemische Biologie, Germany, Fax: (+49) 231-133-2499
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Abstract

A new flexible and efficient methodology for the solid-phase synthesis of lipidated peptides has been developed. The approach is based on the use of previously synthesized building blocks and overcomes the limitations of previously reported methods, since long doubly lipidated peptides can be synthesized by using this route. Furthermore, it was thus possible to prepare a large number of N- and H-Ras peptides bearing a wide range of reporter and/or linking groups—efficient tools for the investigation of biological processes. In terms of efficiency and flexibility this solid-phase method is superior to the solution-phase synthesis. It gives pure peptides in multimilligram amounts within a much shorter time and with superior overall yield.

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