Get access

Corozalic Acid: A Key Okadaic Acid Biosynthetic Precursor with Phosphatase Inhibition Activity

Authors

  • Dr. José G. Napolitano,

    1. Instituto Universitario de Bio-Orgánica “Antonio González”, Universidad de La Laguna, Av. Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife (Spain), Fax: (+34) 922-318571
    Search for more papers by this author
  • Prof. Manuel Norte,

    1. Instituto Universitario de Bio-Orgánica “Antonio González”, Universidad de La Laguna, Av. Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife (Spain), Fax: (+34) 922-318571
    Search for more papers by this author
  • Prof. José Javier Fernández,

    1. Instituto Universitario de Bio-Orgánica “Antonio González”, Universidad de La Laguna, Av. Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife (Spain), Fax: (+34) 922-318571
    Search for more papers by this author
  • Dr. Antonio Hernández Daranas

    1. Instituto Universitario de Bio-Orgánica “Antonio González”, Universidad de La Laguna, Av. Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife (Spain), Fax: (+34) 922-318571
    Search for more papers by this author

Abstract

original image

Toxin precursors: Corozalic acid, a new Ser/Thr protein phosphatase 1 and 2A inhibitor that turned out to be a key metabolic intermediate, was isolated from cultures of the marine dinoflagellate Prorocentrum belizeanum. Detailed spectroscopic analysis and extensive computational calculations revealed its structure and conformational behavior. In addition, a comprehensive picture of the interactions of corozalic acid with PP1 and PP2A is proposed (see figure) on the basis of ab initio, docking and molecular dynamics calculations.

Ancillary