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Aptamer/Nanoparticle-Based Sensitive, Multiplexed Electronic Coding of Proteins and Small Biomolecules through a Backfilling Strategy

Authors

  • Xiaoqing Qian,

    1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (P.R. China), Fax: (+86) 68254000
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  • Prof. Yun Xiang,

    Corresponding author
    1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (P.R. China), Fax: (+86) 68254000
    • Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (P.R. China), Fax: (+86) 68254000
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  • Haixia Zhang,

    1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (P.R. China), Fax: (+86) 68254000
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  • Ying Chen,

    1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (P.R. China), Fax: (+86) 68254000
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  • Prof. Yaqin Chai,

    1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (P.R. China), Fax: (+86) 68254000
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  • Prof. Ruo Yuan

    Corresponding author
    1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (P.R. China), Fax: (+86) 68254000
    • Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (P.R. China), Fax: (+86) 68254000
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Abstract

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Sensing large and small simultaneously: Sensitive and multiplexed analysis of lysozyme and ATP analytes is achieved through target-induced release of aptamers from the DNA duplexes on a sensing surface, followed by backfilling hybridization of the resulting single-stranded DNAs with aptamers conjugated to the electrochemically encoded nanoparticles (see graphic).

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