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Development of Lipid Targeting Raman Probes for In Vivo Imaging of Caenorhabditis elegans

Authors

  • Shobhit Charan,

    1. Research Center For Applied Sciences, Academia Sinica, Taipei−115 (Taiwan), Fax: (+886) 2-2782-6680
    2. Department of Chemistry, National Taiwan University, Taipei 106, (Taiwan), Taiwan International Graduate Program (TIGP), Nanoscience and Technology Program, Academia Sinica, Taipei-115 (Taiwan)
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  • Dr. Fan-Ching Chien,

    1. Research Center For Applied Sciences, Academia Sinica, Taipei−115 (Taiwan), Fax: (+886) 2-2782-6680
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  • Dr. Narendra Singh,

    1. Research Center For Applied Sciences, Academia Sinica, Taipei−115 (Taiwan), Fax: (+886) 2-2782-6680
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  • Dr. Chiung-Wen Kuo,

    1. Research Center For Applied Sciences, Academia Sinica, Taipei−115 (Taiwan), Fax: (+886) 2-2782-6680
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  • Dr. Peilin Chen

    Corresponding author
    1. Research Center For Applied Sciences, Academia Sinica, Taipei−115 (Taiwan), Fax: (+886) 2-2782-6680
    • Research Center For Applied Sciences, Academia Sinica, Taipei−115 (Taiwan), Fax: (+886) 2-2782-6680
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Abstract

A simple, sensitive, and highly specific lipid targeting Raman probe (Nile red coated silver nanoparticles) has been developed to image living nematode Caenorhabditis elegans (C. elegans). Our idea of imaging lipids in C. elegans is to combine the specificity of the fluorescent dye, Nile red, and the highly enhanced Raman scattering on the silver nanoparticles. Our strategy involves the fabrication of a lipid targeting probe, which is incorporated into the intracellular intestinal granules of C. elegans by incubating these worms in the solution containing Raman probes, resulting in an uptake and subsequent incorporation of these Raman probes into the intestinal granule, thus allowing fast visualization of lipid droplets through a conventional confocal imaging technique.

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