Paper-Based Electrochemiluminescent 3D Immunodevice for Lab-on-Paper, Specific, and Sensitive Point-of-Care Testing

Authors

  • Dr. Jixian Yan,

    1. Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (P. R. China), Fax: (+86) 531-82765969
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  • Dr. Lei Ge,

    1. Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (P. R. China), Fax: (+86) 531-82765969
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  • Prof. Xianrang Song,

    1. Cancer Research Center, Shandong Tumor Hospital, Jinan 250117 (P. R. China)
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  • Prof. Mei Yan,

    1. Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (P. R. China), Fax: (+86) 531-82765969
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  • Dr. Shenguang Ge,

    1. Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, University of Jinan, Jinan 250022 (P. R. China)
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  • Prof. Jinghua Yu

    Corresponding author
    1. Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (P. R. China), Fax: (+86) 531-82765969
    • Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (P. R. China), Fax: (+86) 531-82765969
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Abstract

Recent research on microfluidic paper-based analytical devices (μPADs) has shown that paper has great potential for the fabrication of low-cost diagnostic devices for healthcare and environmental monitoring applications. Herein, electrochemiluminescence (ECL) was introduced for the first time into μPADs that were based on screen-printed paper-electrodes. To further perform high-specificity, high-performance, and high-sensitivity ECL on μPADs for point-of-care testing (POCT), ECL immunoassay capabilities were introduced into a wax-patterned 3D paper-based ECL device, which was characterized by SEM, contact-angle measurement, and electrochemical impedance spectroscopy. With the aid of a home-made device-holder, the ECL reaction was triggered at room temperature. By using a typical tris(bipyridine)ruthenium–tri-n-propylamine ECL system, this paper-based ECL 3D immunodevice was applied to the diagnosis of carcinoembryonic antigens in real clinical serum samples. This contribution further expands the number of sensitive and specific detection modes of μPADs.

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