Get access

Kinetic Differentiation between Homo- and Alternating AT DNA by Sterically Restricted Phosphonium Dyes

Authors

  • Dr. Lidija-Marija Tumir,

    1. Division of Organic Chemistry & Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička c. 54, P. O. Box 180, 10002 Zagreb (Croatia)
    Search for more papers by this author
  • Dr. Ivo Crnolatac,

    1. Division of Organic Chemistry & Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička c. 54, P. O. Box 180, 10002 Zagreb (Croatia)
    Search for more papers by this author
  • Prof. Todor Deligeorgiev,

    Corresponding author
    1. Faculty of Chemistry, University of Sofia, J. Bourchier Avenue, 1164, Sofia (Bulgaria)
    • Faculty of Chemistry, University of Sofia, J. Bourchier Avenue, 1164, Sofia (Bulgaria)
    Search for more papers by this author
  • Aleksey Vasilev,

    1. Faculty of Chemistry, University of Sofia, J. Bourchier Avenue, 1164, Sofia (Bulgaria)
    Search for more papers by this author
  • Dr. Stefka Kaloyanova,

    1. Faculty of Chemistry, University of Sofia, J. Bourchier Avenue, 1164, Sofia (Bulgaria)
    Search for more papers by this author
  • Marina Grabar Branilović,

    1. Division of Organic Chemistry & Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička c. 54, P. O. Box 180, 10002 Zagreb (Croatia)
    Search for more papers by this author
  • Dr. Sanja Tomić,

    Corresponding author
    1. Division of Organic Chemistry & Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička c. 54, P. O. Box 180, 10002 Zagreb (Croatia)
    • Division of Organic Chemistry & Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička c. 54, P. O. Box 180, 10002 Zagreb (Croatia)
    Search for more papers by this author
  • Dr. Ivo Piantanida

    Corresponding author
    1. Division of Organic Chemistry & Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička c. 54, P. O. Box 180, 10002 Zagreb (Croatia)
    • Division of Organic Chemistry & Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička c. 54, P. O. Box 180, 10002 Zagreb (Croatia)
    Search for more papers by this author

Abstract

original image

Poly has a tracker: By sterically controlling monomer–dimer equilibria, novel dyes can kinetically differentiate between homo-AT and alternating AT–AT polynucleotides. In this way, the width and steric occupation of the DNA minor groove can be finely probed and can be easily assessed by induced CD signals (see figure).

Ancillary