Structure and Dynamics of Triazole-Linked DNA: Biocompatibility Explained

Authors

  • Dr. André Dallmann,

    Corresponding author
    1. Department of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin (Germany)
    2. Present address: Department of Chemistry, TU München, 85748 Garching (Germany)
    • Department of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin (Germany)
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  • Dr. Afaf H. El-Sagheer,

    1. School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ (UK)
    2. Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez Canal University, Suez, 43721 (Egypt)
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  • Dipl.-Chem. Lars Dehmel,

    1. Department of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin (Germany)
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  • Dr. Clemens Mügge,

    1. Department of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin (Germany)
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  • Prof. Dr. Christian Griesinger,

    1. Department of NMR-Based Structural Biology, Max-Planck Institute for Biophysical Chemistry, 37077 Göttingen (Germany)
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  • Prof. Dr. Nikolaus P. Ernsting,

    1. Department of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin (Germany)
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  • Prof. Dr. Tom Brown

    Corresponding author
    1. School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ (UK)
    • School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ (UK)
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Abstract

original image

Blending in: A triazole-modified DNA duplex is perturbed in structure and dynamics, but this is delocalized over five base pairs. The conformation remains B-DNA and hydrogen bonds between the DNA phosphate oxygen and polymerases can be mimicked by the triazole nitrogen (see figure). The results explain the surprising biocompatibility of triazole-linked DNA.

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