Inside Cover: Selective Two-Step Labeling of Proteins with an Off/On Fluorescent Probe (Chem. Eur. J. 52/2011)

Authors

  • Kazuhisa Hirabayashi,

    1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-4855
    2. CREST, Japan Science and Technology Agency, 3–5 Sanbancho, Chiyoda, Tokyo 102-0075 (Japan)
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  • Dr. Kenjiro Hanaoka,

    1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-4855
    2. CREST, Japan Science and Technology Agency, 3–5 Sanbancho, Chiyoda, Tokyo 102-0075 (Japan)
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  • Dr. Manabu Shimonishi,

    1. Global COE, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan)
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  • Takuya Terai,

    1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-4855
    2. CREST, Japan Science and Technology Agency, 3–5 Sanbancho, Chiyoda, Tokyo 102-0075 (Japan)
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  • Dr. Toru Komatsu,

    1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-4855
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  • Dr. Tasuku Ueno,

    1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-4855
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  • Prof. Dr. Tetsuo Nagano

    Corresponding author
    1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-4855
    2. CREST, Japan Science and Technology Agency, 3–5 Sanbancho, Chiyoda, Tokyo 102-0075 (Japan)
    • Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan), Fax: (+81) 3-5841-4855
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Abstract

original image

Fluorescent Probes In their Full Paper on page 14763 ff., T. Nagano et al. describe the selective two-step labeling of proteins with an off/on fluorescent probe. The probe consists of dichlorofluorescein conjugated with nitrilotriacetic acid (NTA)-Ni2+ as the His-tag recognition site and a 2,4-dinitrophenyl ether moiety, which quenches the probe's fluorescence by photoinduced electron-transfer (PeT) from the excited fluorophore to the 2,4-dinitrophenyl ether and also has reactivity with cysteine. Removal of the 2,4 dinitrophenyl ether quencher by a proximity-enhanced reaction with the cysteine residue of the modified tag results in a marked fluorescence increase.

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