Communication

Ratiometric Fluorescent Signaling of Small Molecule, Environmentally Sensitive Dye Conjugates for Detecting Single-Base Mutations in DNA

  1. Chun-xia Wang,
  2. Dr. Yusuke Sato,
  3. Megumi Kudo,
  4. Dr. Seiichi Nishizawa,
  5. Prof. Dr. Norio Teramae*

Article first published online: 25 JUN 2012

DOI: 10.1002/chem.201200219

Issue

Chemistry - A European Journal

Chemistry - A European Journal

Volume 18, Issue 31, pages 9481–9484, July 27, 2012

Additional Information

How to Cite

Wang, C.-x., Sato, Y., Kudo, M., Nishizawa, S. and Teramae, N. (2012), Ratiometric Fluorescent Signaling of Small Molecule, Environmentally Sensitive Dye Conjugates for Detecting Single-Base Mutations in DNA. Chem. Eur. J., 18: 9481–9484. doi: 10.1002/chem.201200219

Author Information

  1. Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578 (Japan), Fax: (+81) 22-795-6552

*Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578 (Japan), Fax: (+81) 22-795-6552

Publication History

  1. Issue published online: 20 JUL 2012
  2. Article first published online: 25 JUN 2012
  3. Manuscript Received: 20 JAN 2012

Funded by

  • Grant-in-Aid for Scientific Research. Grant Number: 22225003
  • Ministry of Education, Culture, Sports, Science and Technology
  • Grant-in-Aid for Young Scientists. Grant Number: 22850001

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (615K)

Keywords:

  • DNA recognition;
  • fluorescent probes;
  • ligand design;
  • noncovalent interactions;
  • nucleobases
Thumbnail image of graphical abstract

Bind it! A ratiometric fluorescent probe based on conjugation of a DNA-binding ligand with an environmentally sensitive dye to probe hydrophobic grooves of the DNA helix was developed. The resulting conjugate ATMND–DBD can bind to an orphan nucleobase opposite an abasic (AP) site with high selectivity and affinity for pyrimidine bases (see figure).

View Full Article with Supporting Information (HTML) Get PDF (615K)