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Ratiometric Fluorescent Signaling of Small Molecule, Environmentally Sensitive Dye Conjugates for Detecting Single-Base Mutations in DNA

Authors

  • Chun-xia Wang,

    1. Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578 (Japan), Fax: (+81) 22-795-6552
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  • Dr. Yusuke Sato,

    1. Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578 (Japan), Fax: (+81) 22-795-6552
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  • Megumi Kudo,

    1. Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578 (Japan), Fax: (+81) 22-795-6552
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  • Dr. Seiichi Nishizawa,

    1. Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578 (Japan), Fax: (+81) 22-795-6552
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  • Prof. Dr. Norio Teramae

    Corresponding author
    1. Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578 (Japan), Fax: (+81) 22-795-6552
    • Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578 (Japan), Fax: (+81) 22-795-6552
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Abstract

original image

Bind it! A ratiometric fluorescent probe based on conjugation of a DNA-binding ligand with an environmentally sensitive dye to probe hydrophobic grooves of the DNA helix was developed. The resulting conjugate ATMND–DBD can bind to an orphan nucleobase opposite an abasic (AP) site with high selectivity and affinity for pyrimidine bases (see figure).

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