Directed Supramolecular Surface Assembly of SNAP-tag Fusion Proteins

Authors

  • Dr. Dana A. Uhlenheuer,

    1. Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, P. O. Box 513, 5600 MB, Eindhoven (The Netherlands)
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    • Part of the Ph.D. Thesis of D.A.U., ISBN: 978-90-386-2536-2, TU Eindhoven, 2011.

  • Dr. Dorothee Wasserberg,

    1. Molecular nanoFabrication Group, MESA+Institute for Nanotechnology and University of Twente, P. O. Box 217, 7500 AE, Enschede (The Netherlands)
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  • Dr. Christian Haase,

    1. Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, P. O. Box 513, 5600 MB, Eindhoven (The Netherlands)
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  • Dr. Hoang D. Nguyen,

    1. Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, P. O. Box 513, 5600 MB, Eindhoven (The Netherlands)
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  • Jan Hendrik Schenkel,

    1. Organisch-Chemisches Institut, Westfälische Wilhelms-Universität Münster, Corrensstrasse 40, 48149 Münster (Germany)
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  • Prof. Dr. Jurriaan Huskens,

    1. Molecular nanoFabrication Group, MESA+Institute for Nanotechnology and University of Twente, P. O. Box 217, 7500 AE, Enschede (The Netherlands)
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  • Prof. Dr. Bart Jan Ravoo,

    1. Organisch-Chemisches Institut, Westfälische Wilhelms-Universität Münster, Corrensstrasse 40, 48149 Münster (Germany)
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  • Dr. Pascal Jonkheijm,

    Corresponding author
    1. Molecular nanoFabrication Group, MESA+Institute for Nanotechnology and University of Twente, P. O. Box 217, 7500 AE, Enschede (The Netherlands)
    • Molecular nanoFabrication Group, MESA+Institute for Nanotechnology and University of Twente, P. O. Box 217, 7500 AE, Enschede (The Netherlands)
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  • Prof. Dr. Luc Brunsveld

    Corresponding author
    1. Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, P. O. Box 513, 5600 MB, Eindhoven (The Netherlands)
    • Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, P. O. Box 513, 5600 MB, Eindhoven (The Netherlands)
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Abstract

Supramolecular assembly of proteins on surfaces and vesicles was investigated by site-selective incorporation of a supramolecular guest element on proteins. Fluorescent proteins were site-selectively labeled with bisadamantane by SNAP-tag technology. The assembly of the bisadamantane functionalized SNAP-fusion proteins on cyclodextrin-coated surfaces yielded stable monolayers. The binding of the fusion proteins is specific and occurs with an affinity in the order of 106M−1 as determined by surface plasmon resonance. Reversible micropatterns of the fusion proteins on micropatterned cyclodextrin surfaces were visualized by using fluorescence microscopy. Furthermore, the guest-functionalized proteins could be assembled out of solution specifically onto the surface of cyclodextrin vesicles. The SNAP-tag labeling of proteins thus allows for assembly of modified proteins through a host–guest interaction on different surfaces. This provides a new strategy in fabricating protein patterns on surfaces and takes advantage of the high labeling efficiency of the SNAP-tag with designed supramolecular elements.

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