Fast Fluorescence Switching within Hydrophilic Supramolecular Assemblies

Authors

  • Janet Cusido,

    1. Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida, 33146-0431 (USA), Fax: (+1) 305-284-4571
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  • Mutlu Battal,

    1. Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida, 33146-0431 (USA), Fax: (+1) 305-284-4571
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  • Dr. Erhan Deniz,

    1. Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida, 33146-0431 (USA), Fax: (+1) 305-284-4571
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  • Dr. Ibrahim Yildiz,

    1. Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida, 33146-0431 (USA), Fax: (+1) 305-284-4571
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  • Prof. Salvatore Sortino,

    Corresponding author
    1. Laboratory of Photochemistry, Department of Drug Sciences, University of Catania, Viale Andrea Doria 6, I-95125 Catania (Italy), Fax: (+39) 095-580138
    • Laboratory of Photochemistry, Department of Drug Sciences, University of Catania, Viale Andrea Doria 6, I-95125 Catania (Italy), Fax: (+39) 095-580138
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  • Prof. Françisco M. Raymo

    Corresponding author
    1. Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida, 33146-0431 (USA), Fax: (+1) 305-284-4571
    • Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, Florida, 33146-0431 (USA), Fax: (+1) 305-284-4571
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Abstract

We designed a supramolecular strategy to modulate fluorescence in water under optical control. It is based on the entrapment of fluorophore–photochrome dyads within the hydrophobic interior of an amphiphilic polymer. The polymeric envelope around the dyads protects them from the aqueous environment, while imposing hydrophilic character on the overall supramolecular construct. In the resulting assemblies, the photochromic component can be operated reversibly on a microsecond timescale under the influence of ultraviolet stimulations. In turn, the reversible transformations control the emission intensity of the adjacent fluorophore. As a result, the fluorescence of such nanostructured constructs can be photomodulated for hundreds of cycles in water with microsecond switching speeds. Thus, our protocol for fast fluorescence switching in aqueous solutions can eventually lead to the realization of functional probes for the investigation of biological samples.

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