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Turn-On Fluorescence Sensor Based on Single-Walled-Carbon-Nanohorn–Peptide Complex for the Detection of Thrombin

Authors

  • Shuyun Zhu,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, Jilin 130022 (P.R. China), Fax: (+86) 431-85262747
    2. Shandong Provincial Key Laboratory of Life-Organic Analysis, College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, Shandong 273165 (P.R. China)
    3. Graduate University of the Chinese Academy of Sciences, Chinese Academy of Sciences Changchun, Beijing 100039 (P.R. China)
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  • Zhongyuan Liu,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, Jilin 130022 (P.R. China), Fax: (+86) 431-85262747
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  • Lianzhe Hu,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, Jilin 130022 (P.R. China), Fax: (+86) 431-85262747
    2. Graduate University of the Chinese Academy of Sciences, Chinese Academy of Sciences Changchun, Beijing 100039 (P.R. China)
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  • Yali Yuan,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, Jilin 130022 (P.R. China), Fax: (+86) 431-85262747
    2. Graduate University of the Chinese Academy of Sciences, Chinese Academy of Sciences Changchun, Beijing 100039 (P.R. China)
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  • Prof. Guobao Xu

    Corresponding author
    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, Jilin 130022 (P.R. China), Fax: (+86) 431-85262747
    • State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, Jilin 130022 (P.R. China), Fax: (+86) 431-85262747===

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Abstract

Proteases play a central role in several widespread diseases. Thus, there is a great need for the fast and sensitive detection of various proteolytic enzymes. Herein, we have developed a carbon nanotube (CNT)-based protease biosensing platform that uses peptides as a fluorescence probe for the first time. Single-walled carbon nanohorns (SWCNHs) and thrombin were used to demonstrate this detection strategy. SWCNHs can adsorb a fluorescein-based dye (FAM)-labeled peptide (FAM-pep) and quench the fluorescence of FAM. In contrast, thrombin can cleave FAM-pep on SWCNHs and recover the fluorescence of FAM, which allows the sensitive detection of thrombin. This biosensor has a high sensitivity and selectivity toward thrombin, with a detection limit of 100 pM.

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