Get access

Rapid and Specific Post-Synthesis Modification of DNA through a Biocompatible Condensation of 1,2-Aminothiols with 2-Cyanobenzothiazole

Authors

  • Dr. Yunfeng Cheng,

    1. Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dr. Hanjing Peng,

    1. Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Dr. Weixuan Chen,

    1. Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    Search for more papers by this author
  • Dr. Nanting Ni,

    1. Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    Search for more papers by this author
  • Dr. Bowen Ke,

    1. Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    Search for more papers by this author
  • Dr. Chaofeng Dai,

    Corresponding author
    1. Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    • Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    Search for more papers by this author
  • Prof. Binghe Wang

    Corresponding author
    1. Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    • Department of Chemistry, Center for Diagnostics and Therapeutics, and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098 (USA), Fax: (+1) 404-413-5543
    Search for more papers by this author

Abstract

Post-synthesis modification of DNA is an important way of functionalizing DNA molecules. Herein, we describe a method that first enzymatically incorporates a cyanobenzothiazole (CBT)-modified thymidine. The side-chain handle CBT can undergo a rapid and site-specific cyclization reaction with 1,2-aminothiols to afford DNA functionalization in aqueous solution. Another key advantage of this method is the formation of a single stereo/regioisomer in the process, which allows for precise control of DNA modification to yield a single component for aptamer selection work and other applications.

Get access to the full text of this article

Ancillary