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A New Signal-On Photoelectrochemical Biosensor Based on a Graphene/Quantum-Dot Nanocomposite Amplified by the Dual-Quenched Effect of Bipyridinium Relay and AuNPs

Authors

  • Dr. Xiaoru Zhang,

    1. Key Laboratory of Biochemical Analysis, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (P.R. China), Fax: (+86) 532-84022750
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  • Yunpeng Xu,

    1. Key Laboratory of Biochemical Analysis, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (P.R. China), Fax: (+86) 532-84022750
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  • Prof. Yanqiang Yang,

    1. Center for Condensed Matter Science and Technology, Department of Physics, Harbin Institute of Technology, Harbin 150001 (P.R. China)
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  • Xia Jin,

    1. Key Laboratory of Biochemical Analysis, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (P.R. China), Fax: (+86) 532-84022750
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  • Sujuan Ye,

    1. Key Laboratory of Biochemical Analysis, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (P.R. China), Fax: (+86) 532-84022750
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  • Prof. Shusheng Zhang,

    Corresponding author
    1. Key Laboratory of Biochemical Analysis, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (P.R. China), Fax: (+86) 532-84022750
    • Key Laboratory of Biochemical Analysis, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (P.R. China), Fax: (+86) 532-84022750===

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  • Dr. Lilin Jiang

    1. Center for Condensed Matter Science and Technology, Department of Physics, Harbin Institute of Technology, Harbin 150001 (P.R. China)
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Abstract

A new photoelectrochemical (PEC) biosensor was developed by using carboxyl-functionalized graphene and CdSe nanoparticles. This sensitive interface was then successfully applied to detection of thrombin based on the dual-quenched effect of PEC nanoparticle, which relied on the electron transfer of a bipyridinium relay and energy transfer of AuNPs. After recognition with an aptamer, the PEC nanoparticle was removed and a signal-on PEC biosensor was obtained. Moreover, the bio-barcode technique used in the preparation of PEC nanoparticle could avoid cross-reaction and enhances the sensitivity. Taking advantages of the various methods mentioned above, the sensitivity could be easily enhanced. In addition, in this work we also investigated graphene that was modified with different functional groups and AuNPs of different particle sizes. Under optimal conditions, a detection limit of 5.9×10−15M was achieved. With its simplicity, selectivity, and sensitivity, this strategy shows great promise for the fabrication of highly efficient PEC biosensors.

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