Ratiometric Detection of Viscosity Using a Two-Photon Fluorescent Sensor

Authors

  • Fei Liu,

    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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  • Dr. Tong Wu,

    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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  • Jianfang Cao,

    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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  • Shuang Cui,

    1. School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China)
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  • Dr. Zhigang Yang,

    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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  • Dr. Xinxin Qiang,

    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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  • Dr. Shiguo Sun,

    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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  • Dr. Fengling Song,

    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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  • Dr. Jiangli Fan,

    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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  • Jingyun Wang,

    Corresponding author
    1. School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China)
    • School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China)
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  • Prof. Xiaojun Peng

    Corresponding author
    1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
    • State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-Tech District, Dalian 116024 (P.R. China), Fax: (+86) 411-84986306
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Abstract

original image

In the thick of it: A carbazole-based cyanine derivative has been used as a ratiometric fluorescent sensor to detect mitochondrial viscosities over the range 1–950 cP within live cells and living tissues by using two-photon microscopy. Its success is based on the fast- and low-barrier rotation around its methine bond (see figure) and the huge increase of the fluorescence quantum yield (QY) from the loss of mobility in viscous media.

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