Get access
Advertisement

Surface-Assisted Laser Desorption/Ionization Mass Spectrometric Detection of Biomolecules by Using Functional Single-Walled Carbon Nanohorns as the Matrix

Authors

  • Rongna Ma,

    1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China), Fax: (+86) 25-8359-3593
    Search for more papers by this author
  • Minghua Lu,

    1. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (China), Fax: (+852) 3411-7348
    Search for more papers by this author
  • Lin Ding,

    1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China), Fax: (+86) 25-8359-3593
    Search for more papers by this author
  • Prof. Huangxian Ju,

    Corresponding author
    1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China), Fax: (+86) 25-8359-3593
    • State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (P.R. China), Fax: (+86) 25-8359-3593
    Search for more papers by this author
  • Prof. Zongwei Cai

    Corresponding author
    1. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (China), Fax: (+852) 3411-7348
    • Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR (China), Fax: (+852) 3411-7348
    Search for more papers by this author

Abstract

A surface-assisted laser desorption/ionization time-of-flight mass spectrometric (SALDI-TOF MS) method was developed for the analysis of small biomolecules by using functional single-walled carbon nanohorns (SWNHs) as matrix. The functional SWNHs could transfer energy to the analyte under laser irradiation for accelerating its desorption and ionization, which led to low matrix effect, avoided fragmentation of the analyte, and provided high salt tolerance. Biomolecules including amino acids, peptides, and fatty acids could successfully be analyzed with about 3- and 5-fold higher signals than those obtained using conventional matrix. By integrating the advantages of SWNHs and the recognition ability of aptamers, a selective approach was proposed for simultaneous capture, enrichment, ionization, and MS detection of adenosine triphosphate (ATP). This method showed a greatly improved detection limit (1.0 μM) for the analysis of ATP in complex biological samples. This newly designed protocol not only opened a new application of SWNHs, but also offered a new technique for selective MS analysis of biomolecules based on aptamer recognition systems.

Get access to the full text of this article

Ancillary