Sensitive and Convenient Detection of microRNAs Based on Cascade Amplification by Catalytic DNAzymes

Authors

  • Dr. Tian Tian,

    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
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    • These authors contributed equally to this work.

  • Heng Xiao,

    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
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    • These authors contributed equally to this work.

  • Zhengan Zhang,

    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
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  • Yuelin Long,

    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
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  • Shuang Peng,

    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
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  • Dr. Shaoru Wang,

    Corresponding author
    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
    • College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
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  • Prof. Dr. Xiang Zhou,

    Corresponding author
    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
    2. State Key Laboratory of Natural and Biomimetic Drugs, Peking University (P. R. China)
    • College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072 (P. R. China), Fax: (+) 86-27-68756663
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  • Dr. Songmei Liu,

    1. Zhongnan Hosptial, Wuhan University (P. R. China)
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  • Prof. Xin Zhou

    1. Zhongnan Hosptial, Wuhan University (P. R. China)
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Abstract

original image

On target: We have developed two cascade amplification strategies that combine duplex specific nuclease (DSN) amplicon with either G-quadruplex-based DNA peroxidase or 8–17 DNAzyme amplicon for miRNA detection. In this way, sensitive and convenient detection of miRNAs was achieved. In the DNA peroxidase-based system, a visual color change could be observed in the presence of target miRNAs (see scheme).

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