Communication

Simultaneous Imaging of Mitochondria and Lysosomes by Using Two-Photon Fluorescent Probes

  1. Ji Hee Han1,
  2. Sun Keun Park2,
  3. Chang Su Lim1,
  4. Min Kyu Park1,
  5. Hyung Joong Kim2,
  6. Prof. Hwan Myung Kim2,*,
  7. Prof. Bong Rae Cho1,*

Article first published online: 30 OCT 2012

DOI: 10.1002/chem.201203452

Issue

Chemistry - A European Journal

Chemistry - A European Journal

Volume 18, Issue 48, pages 15246–15249, November 26, 2012

Additional Information

How to Cite

Han, J. H., Park, S. K., Lim, C. S., Park, M. K., Kim, H. J., Kim, H. M. and Cho, B. R. (2012), Simultaneous Imaging of Mitochondria and Lysosomes by Using Two-Photon Fluorescent Probes. Chem. Eur. J., 18: 15246–15249. doi: 10.1002/chem.201203452

Author Information

  1. 1

    Department of Chemistry, Korea University, 1-Anamdong, Seoul, 136-701 (Korea), Fax: (+82) 2-3290-3544

  2. 2

    Division of Energy Systems Research, Ajou University, Suwon, 443-749 (Korea), Fax: (+82) 31-219-1615

*Division of Energy Systems Research, Ajou University, Suwon, 443-749 (Korea), Fax: (+82) 31-219-1615

Publication History

  1. Issue published online: 19 NOV 2012
  2. Article first published online: 30 OCT 2012
  3. Manuscript Received: 27 SEP 2012

Funded by

  • KHT R&D Project
  • Ministry of Health & Welfare. Grant Number: A111182
  • National Research Foundation (NRF)
  • Korean Government. Grant Number: 2011-0028663

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Keywords:

  • fluorescence;
  • fluorescent probes;
  • imaging agents;
  • lysosomes;
  • mitochondria
Thumbnail image of graphical abstract

Dual-color imaging: Two-photon probes for lysosomes (BLT-blue) and mitochondria (FMT-green) that can be excited by 750 nm femtosecond laser pulses and emit at λ=400–450 and 550–600 nm, respectively, are reported. They allow simultaneous dual-color imaging of lysosomes and mitochondria in live cells and tissues for a long period of time with minimum interference from pH, photobleaching, or cytotoxicity (see scheme).

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