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Keywords:

  • fluorescence;
  • fluorescent probes;
  • imaging agents;
  • lysosomes;
  • mitochondria
Thumbnail image of graphical abstract

Dual-color imaging: Two-photon probes for lysosomes (BLT-blue) and mitochondria (FMT-green) that can be excited by 750 nm femtosecond laser pulses and emit at λ=400–450 and 550–600 nm, respectively, are reported. They allow simultaneous dual-color imaging of lysosomes and mitochondria in live cells and tissues for a long period of time with minimum interference from pH, photobleaching, or cytotoxicity (see scheme).