Get access

Bioluminescent Microcapsules: Applications in Activating a Photosensitizer

Authors

  • Dr. Jie Zhao,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    Search for more papers by this author
  • Dr. Jinbo Fei,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    Search for more papers by this author
  • Dr. Liang Gao,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    Search for more papers by this author
  • Wei Cui,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    Search for more papers by this author
  • Dr. Yang Yang,

    1. National Center for Nanoscicence and Technology, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
    Search for more papers by this author
  • Dr. Anhe Wang,

    1. National Center for Nanoscicence and Technology, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
    Search for more papers by this author
  • Prof. Dr. Junbai Li

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    2. National Center for Nanoscicence and Technology, Beijing, 100190 (P.R. China), Fax: (+86) 10-82612629
    • Beijing National Laboratory for Molecular Sciences (BNLMS), Key Lab of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190 (P.R. China)
    Search for more papers by this author

Abstract

Bioluminescent microcapsules uploading D-luciferin have been fabricated by using the covalent assembly of firefly luciferase and alginate dialdehyde through a layer-by-layer technique. Such assembled microcapsules can produce visible light in the region of 520–680 nm, which can activate the photosensitizers rose bengal (RB) and hypocrellin B (HB) after adding ATP. The microcapsules uploading photosensitizers (RB or HB) have an obvious property to prevent the proliferation of tumor cells in the dark. The assembled bioluminescent microcapsules can be potentially used as photon donors for bioimaging, ATP detection, and photodynamic therapy.

Get access to the full text of this article

Ancillary