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A Peptide Nucleic Acid (PNA) Heteroduplex Probe Containing an Inosine–Cytosine Base Pair Discriminates a Single-Nucleotide Difference in RNA

Authors

  • Dr. Katsuhiko Matsumoto,

    1. Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011 (Japan), Fax: (+81) 774-38-3516
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  • Dr. Eiji Nakata,

    1. Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011 (Japan), Fax: (+81) 774-38-3516
    2. Japan Science and Technology Agency (JST-CREST), Uji, Kyoto, 611-0011 (Japan)
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  • Tomoki Tamura,

    1. Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011 (Japan), Fax: (+81) 774-38-3516
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  • Prof. Dr. Isao Saito,

    1. NEWCAT Institute, School of Engineering, Nihon University, Koriyama, Fukushima, 963-8642 (Japan)
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  • Dr. Yasunori Aizawa,

    1. Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501 (Japan)
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  • Prof. Dr. Takashi Morii

    Corresponding author
    1. Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011 (Japan), Fax: (+81) 774-38-3516
    2. Japan Science and Technology Agency (JST-CREST), Uji, Kyoto, 611-0011 (Japan)
    • Institute of Advanced Energy, Kyoto University, Uji, Kyoto, 611-0011 (Japan), Fax: (+81) 774-38-3516
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Abstract

Selective discrimination of a single-nucleotide difference in single-stranded DNA or RNA remains a challenge with conventional DNA or RNA probes. A peptide nucleic acid (PNA)-derived probe, in which PNA forms a pseudocomplementary heteroduplex with inosine-containing DNA or RNA, effectively discriminates a single-nucleotide difference in a closely related group of sequences of single-stranded DNA and/or RNA. The pseudocomplementary PNA heteroduplex is easily converted to a fluorescent probe that distinctively detects a member of highly homologous let-7 microRNAs.

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