Forming Lipid Bilayer Membrane Arrays on Micropatterned Polyelectrolyte Film Surfaces

Authors

  • Ying Zhang,

    1. School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001 (P.R. China)
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  • Lei Wang,

    1. School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001 (P.R. China)
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  • Xuejing Wang,

    1. School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001 (P.R. China)
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  • Guodong Qi,

    1. School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001 (P.R. China)
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  • Prof. Xiaojun Han 

    Corresponding author
    1. School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001 (P.R. China)
    2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001 (P.R. China)
    • School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001 (P.R. China)
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Abstract

A novel method of forming lipid bilayer membrane arrays on micropatterned polyelectrolyte film surfaces is introduced. Polyelectrolyte films were fabricated by the layer-by-layer technique on a silicon oxide surface modified with a 3-aminopropyltriethoxysilane (APTES) monolayer. The surface pKa value of the APTES monolayer was determined by cyclic voltammetry to be approximately 5.61, on the basis of which a pH value of 2.0 was chosen for layer-by-layer assembly. Micropatterned polyelectrolyte films were obtained by deep-UV (254 nm) photolysis though a mask. Absorbed fluorescent latex beads were used to visualize the patterned surfaces. Lipid bilayer arrays were fabricated on the micropatterned surfaces by immersing the patterned substrates into a solution containing egg phosphatidylcholine vesicles. Fluorescence recovery after photobleaching studies yielded a lateral diffusion coefficient for probe molecules of 1.31±0.17 μm2 s−1 in the bilayer region, and migration of the lipid NBD PE in bilayer lipid membrane arrays was observed in an electric field.

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