Biofunctionalization of Multiwalled Carbon Nanotubes by Irradiation of Electropolymerized Poly(pyrrole–diazirine) Films

Authors

  • Dr. Vladislav Papper,

    1. NTU CREATE, Research Wing #02-06, Nanyang Technological University, 138602 (Singapore)
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  • Dr. Karine Gorgy,

    1. Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, CNRS, Université Joseph Fourier, 38041 Grenoble (France), Fax: (+33) 456520805
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  • Dr. Kamal Elouarzaki,

    1. Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, CNRS, Université Joseph Fourier, 38041 Grenoble (France), Fax: (+33) 456520805
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  • Ayrine Sukharaharja,

    1. NTU CREATE, Research Wing #02-06, Nanyang Technological University, 138602 (Singapore)
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  • Dr. Serge Cosnier,

    Corresponding author
    1. Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, CNRS, Université Joseph Fourier, 38041 Grenoble (France), Fax: (+33) 456520805
    • Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, CNRS, Université Joseph Fourier, 38041 Grenoble (France), Fax: (+33) 456520805

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  • Prof. Robert S. Marks 

    1. NTU CREATE, Research Wing #02-06, Nanyang Technological University, 138602 (Singapore)
    2. The Department of Biotechnology Engineering, The National Institute for Biotechnology in the Negev, The Ilse Kats Institute for Nanoscale Science and Technology, The Ben Gurion University of the Negev, Beer Sheva, 84105 (Israel)
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Abstract

A photoactivatable poly(pyrrole–diazirine) film was synthesized and electropolymerized as a versatile tool for covalent binding of laccase and glucose oxidase on multiwalled carbon nanotube coatings and Pt, respectively. Irradiation of the functionalized nanotubes allowed photochemical grafting of laccase and its subsequent direct electrical wiring, as illustrated by the electrocatalytic reduction of oxygen. Moreover, covalent binding of glucose oxidase as model enzyme, achieved by UV activation of electropolymerized pyrrole–diazirine, allowed a glucose biosensor to be realized. This original method to graft biomolecules combines electrochemical and photochemical techniques. The simplicity of this new method allows it to be extended easily to other biological systems.

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