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A Self-Assembled Nanohybrid Composed of Fluorophore–Phenylamine Nanorods and Ag Nanocrystals: Energy Transfer, Wavelength Shift of Fluorescence and TPEF Applications for Live-Cell Imaging

Authors

  • Prof. Lin Kong,

    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
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  • Prof. Jia-xiang Yang,

    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
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  • Prof. Sheng-li Li,

    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
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  • Dr. Qiong Zhang,

    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
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  • Prof. Zhao-ming Xue,

    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
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  • Prof. Hong-ping Zhou,

    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
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  • Prof. Jie-ying Wu,

    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
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  • Prof. Bao-kang Jin,

    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
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  • Prof. Yu-peng Tian 

    Corresponding author
    1. Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279
    2. State Key Laboratory of Crystal Materials, Shandong University, 27 Shan-Da South Road, Jinan, (P. R. China)
    • Department of Chemistry, Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Anhui University, 111 Jiu-Long Road, Hefei, 230039 (P.R. China), Fax: (+86) 551-63861279

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  •  TPEF=Two-photon-excited fluorescence

Abstract

A fluorophore–phenylamine derivative (L) has been coupled with silver nanocrystals (NCs) to construct an L–Ag nanohybrid. Owing to synergic effects of the L and Ag components, the exciton–plasmon interactions between L and Ag increase the strength of the donor–acceptor interaction within the nanohybrid, a fact that results in an energy-transfer process and further brings about a dramatic redshift of single-photon absorption and fluorescence, and a decreased fluorescence FL lifetime. The coupling effect also leads to enhancement of a series of nonlinear optical properties, including two-photon-excited fluorescence (TPEF), two-photon-absorption (TPA) cross section (δ), two-photon-absorption coefficient (β), nonlinear refractive index (γ), and third order nonlinear optical susceptibility (χ(3)). The enhanced two-photon fluorescence of the nanohybrid is proven to be potentially useful for two-photon microscopy of live cells, such as HepG2. Moreover, cytotoxicity tests show that the low-micromolar concentrations of the nanohybrid do not cause significant reduction in cell viability over a period of at least 24 h and should be safe for further biological studies.

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