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Regulation of B–Z Conformational Transition and Complex Formation with a Z-Form Binding Protein by Introduction of Constraint to Double-Stranded DNA by using a DNA Nanoscaffold

Authors

  • Prof. Dr. Masayuki Endo,

    Corresponding author
    1. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan), Fax: (+81) 753-3670
    2. CREST (Japan) Science and Technology Corporation (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075 (Japan)
    • Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan), Fax: (+81) 753-3670

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  • Masahiro Inoue,

    1. Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)
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  • Dr. Yuki Suzuki,

    1. Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)
    2. CREST (Japan) Science and Technology Corporation (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075 (Japan)
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  • Chigusa Masui,

    1. Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)
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  • Dr. Hironobu Morinaga,

    1. Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)
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  • Kumi Hidaka,

    1. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan), Fax: (+81) 753-3670
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  • Prof. Dr. Hiroshi Sugiyama

    Corresponding author
    1. Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan), Fax: (+81) 753-3670
    2. Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan)
    3. CREST (Japan) Science and Technology Corporation (JST), Sanbancho, Chiyoda-ku, Tokyo 102-0075 (Japan)
    • Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan), Fax: (+81) 753-3670

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Abstract

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B–Z transition: A constrained and rotatable double-stranded DNA, in which the rotational freedom was controlled by its placement into a DNA nanoscaffold, was designed (see scheme). The Z α β protein bound preferentially to the rotatable strand rather than the constrained strand, even using the same CG sequence.

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