C5-Functionalized DNA, LNA, and α-L-LNA: Positional Control of Polarity-Sensitive Fluorophores Leads to Improved SNP-Typing (pages 3157–3165)
Dr. Michael E. Østergaard, Dr. Pawan Kumar, Bharat Baral, Dale C. Guenther, Brooke A. Anderson, Prof. F. Marty Ytreberg, Dr. Lee Deobald, Prof. Andrzej J. Paszczynski, Prof. Pawan K. Sharma and Prof. Patrick J. Hrdlicka
Article first published online: 15 FEB 2011 | DOI: 10.1002/chem.201002109
SNP discrimination with LNA: Single-nucleotide polymorphisms (SNPs) are discriminated by using DNA monomer X or the corresponding C5-functionalized locked nucleic acid (LNA) and α-L-LNA monomers Y and Z. Improved positional control of the polarity-sensitive pyrene label results in larger hybridization-induced increases in fluorescence intensity, formation of brightly fluorescent duplexes upon hybridization to complementary nucleic acids, and efficient SNP discrimination.