Fluorogenic Stereochemical Probes for Transaldolases (pages 893–899)
Eva González-García, Virgil Helaine, Gérard Klein, Melanie Schuermann, Georg A. Sprenger, Wolf-Dieter Fessner and Jean-Louis Reymond
Version of Record online: 11 FEB 2003 | DOI: 10.1002/chem.200390110
A fluorescence assay for probing the activity and stereoselectivity of transaldolases, which catalyze the stereoselective aldol coupling between dihydroxyacetone and α-hydroxyaldehydes, is demonstrated. It is based on synthetic probes that undergo selective retroaldolization with the enzymes to form a fluorescent product. These probes comprise the simple fructose analogue 1 and the stereoisomeric pair 2/3, which is used to measure the fructo/tagato-stereoselectivity of transaldolases.