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Keywords:

  • simulated-moving-bed (SMB);
  • enantiomer separation;
  • chiral stationary phases;
  • tert-leucine;
  • polysaccharide;
  • cellulose;
  • amylose

Abstract

The enantiomers of two different derivatives of tert-leucine were separated by continuous chromatography on chiral stationary phases applying the simulated moving bed technique. About 1 kg of racemic N-carbobenzoxy-tert-leucine was resolved on the cellulose-based phase Chiralcel OD using a mixture of heptane/ethanol and 0.1% of trifluoroacetic acid modifier as the mobile phase, while 520 g of the N-Boc-tert-leucine-benzylester was resolved on the amylose-based phase Chiralpak AD with a mixture of heptane/2-propanol as the mobile phase. In both instances the corresponding enantiomers were obtained in high yield and high optical purity. Chirality 14:313–317, 2002. © 2002 Wiley-Liss, Inc.