Enantiomeric analysis of amino acids by using comprehensive two-dimensional gas chromatography
Article first published online: 17 JAN 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 19, Issue 3, pages 228–234, March 2007
How to Cite
Junge, M., Huegel, H. and Marriott, P. J. (2007), Enantiomeric analysis of amino acids by using comprehensive two-dimensional gas chromatography. Chirality, 19: 228–234. doi: 10.1002/chir.20371
- Issue published online: 29 JAN 2007
- Article first published online: 17 JAN 2007
- Manuscript Accepted: 30 NOV 2006
- Manuscript Received: 8 AUG 2006
- Australian Research Council. Grant Number: DP346613
- amino acids;
- enantioselective separation;
- chiral columns
The chiral separation of amino acids (AA) derivatised with ethyl chloroformate by using comprehensive two-dimensional gas chromatography is reported. A commercially available enantioselective capillary column (Chirasil-l-Val) has been tested as first-dimension column. Two nonenantioselective stationary phases (BPX50 and BP1) with different column lengths were combined with the enantioselective column, which represent chiral/polar and chiral/low-polarity column sets, respectively. These column sets were evaluated to determine the most useful column combination to provide improved separation efficiency of enantioselective AA analysis. Separations of AA mixtures derivatised either as their N-trifluoroacetyl methyl esters or with methyl chloroformate, performed on a chiral/low-polarity column set, are also shown. The method was demonstrated for chiral analysis of AAs in different beer samples. The major AA in the beer samples was proline with amounts ranging from around 65–95% with minor contents of glycine and the l-enantiomers of alanine, valine, leucine, and isoleucine. Small amounts of d-alanine, at about 1, 1.5, and 15% were detected in the three samples. Chirality, 2007. © 2007 Wiley-Liss, Inc.