We report on the experimental-theoretical analysis of degenerated two-photon absorption circular-linear dichroism (2PA-CLD) in (S)-(−)-1,1′-bi(2-naphthol) and (R)-(+)-1,1′-bi(2-naphthol) dissolved in THF. 2PA-CLD reveals similarities and differences between optical polarization states for the different enantiomers. These results unveil the potential applications of 2PA-CLD for the study and identification of chiral molecules with active and pivotal role in biological systems and the systematic design of chiral photonic structures. Chirality, 2010. © 2010 Wiley-Liss, Inc.