HPLC–CD selectivity assay for alcohol dehydrogenases

Authors

  • Melissa Hamzic,

    1. Institut für Bioorganische Chemie Heinrich-Heine-Universität Düsseldorf im Forschungszentrum, Jülich, Stetternicher Forst, Geb. 15.8, D-52426 Jülich, Germany
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  • Jörg Pietruszka,

    Corresponding author
    1. Institut für Bioorganische Chemie Heinrich-Heine-Universität Düsseldorf im Forschungszentrum, Jülich, Stetternicher Forst, Geb. 15.8, D-52426 Jülich, Germany
    • Institut für Bioorganische Chemie der Heinrich-Heine Universität im Forschungszentrum Jülich, Germany

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  • Diana Sandkuhl

    1. Institut für Bioorganische Chemie Heinrich-Heine-Universität Düsseldorf im Forschungszentrum, Jülich, Stetternicher Forst, Geb. 15.8, D-52426 Jülich, Germany
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  • Contribution to the Proceedings of the 22nd International Symposium on Chirality [ISCD 22]

Abstract

Enantioselective reductions are a key to successful target-oriented syntheses. Finding the most suitable conditions is often a tedious work that is especially hampered by the time-consuming analytical investigation. A possible solution is the combined use of high-performance liquid chromatography and circular dichroism to find a suitable system for providing enantiomerically pure alcohols. This investigation led to an efficient protocol for the alcohol dehydrogenase-catalyzed reduction of 1-phenyl-2-propyn-3-trimethylsilyl-1-on (). Chirality, 2011. © 2011Wiley Periodicals, Inc.

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