Stereoselective distribution of acenocoumarol enantiomers in human plasma: Chiral chromatographic analysis of the ultrafiltrates
Article first published online: 21 SEP 2004
Copyright © 1993 Wiley-Liss, Inc.
Volume 5, Issue 5, pages 346–349, 1993
How to Cite
Fitos, I., Visy, J., Simonyi, M. and Hermansson, J. (1993), Stereoselective distribution of acenocoumarol enantiomers in human plasma: Chiral chromatographic analysis of the ultrafiltrates. Chirality, 5: 346–349. doi: 10.1002/chir.530050513
- Issue published online: 21 SEP 2004
- Article first published online: 21 SEP 2004
- Manuscript Accepted: 24 DEC 1992
- Manuscript Received: 19 OCT 1992
- stereoselective protein binding;
- chiral liquid chromatography;
- human serum albumin;
- α1-acid glycoprotein;
Stereoselectivities for the binding of rac-acenocoumarol to human serum albumin (HSA), α1-acid glycoprotein (AGP), and human plasma were determined by chiral HPLC analysis of the ultrafiltrates on a Chiral-AGP column. The results confirmed the previously detected inverse stereoselectivities; for HSA the ratio of the enantiomeric constants was KR/KS ∼ 2, while for AGP it was KR/KS ∼ 0.3. In plasma the contribution of HSA dominates, although in pathological states, elevated AGP levels may compensate for stereoselective distribution. © 1993 Wiley-Liss, Inc.