Chemie Ingenieur Technik

Cover image for Vol. 85 Issue 1‐2

Special Issue: Single-Use Technology

February, 2013

Volume 85, Issue 1-2

Pages 1–211

Issue edited by: Regine Eibl, Dieter Eibl

  1. Titelbild

    1. Top of page
    2. Titelbild
    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. You have free access to this content
      Titelbild Chem. Ing. Tech. 1-2/2013 (page 1)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390001

  2. Innentitelbild

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    2. Titelbild
    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
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      Innentitelbild Chem. Ing. Tech. 1-2/2013 (page 2)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390009

  3. Editorial

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    2. Titelbild
    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
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      Single-Use Technology in Modern Biotechnological Processes (page 3)

      Prof. Dr. Dieter Eibl and Prof. Dr. Regine Eibl

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390010

  4. Inhalt

    1. Top of page
    2. Titelbild
    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
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      Inhalt: Chem. Ing. Tech. 1-2/2013 (pages 4–8)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390011

  5. Aus den Gesellschaften

    1. Top of page
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    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. DECHEMA: Chem. Ing. Tech. 1-2/2013 (pages 10–16)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390000

    2. GDCh: Chem. Ing. Tech. 1-2/2013 (pages 17–18)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390002

  6. Personalien

    1. Top of page
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    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. Personalien: Chem. Ing. Tech. 1-2/2013 (page 19)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390003

  7. Forum

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    4. Editorial
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    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. Forum: Chem. Ing. Tech. 1-2/2013 (pages 20–22)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390004

  8. Essays

    1. Top of page
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    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. Single-Use Technologies for Biopharmaceutical Production: Report from the Working Group Bioprocess Technology – Upstream Processing (pages 23–25)

      Dr. Wolfram Meusel, Jörg Kauling and Christian Löffelholz

      Article first published online: 13 DEC 2012 | DOI: 10.1002/cite.201200217

      No abstract.

    2. Interlaboratory Test for Detection of Cytotoxic Leachables arising from Single-Use Bags (pages 26–28)

      Nina Steiger and Prof. Dr. Ing. Regine Eibl

      Article first published online: 3 DEC 2012 | DOI: 10.1002/cite.201200171

      No abstract.

      Corrected by:

      Corrigendum: Interlaboratory Test for Detection of Cytotoxic Leachables arising from Single-Use Bags

      Vol. 85, Issue 4, 396, Article first published online: 22 MAR 2013

    3. Single-Use Bags for Sampling and Storage Studies – Reflecting the State of the Art (pages 29–31)

      Jens Rumsfeld and Jens Kubischik

      Article first published online: 8 JAN 2013 | DOI: 10.1002/cite.201200232

      No abstract.

    4. New Challenges in Project Management for Biopharmaceutical Production (pages 32–33)

      Dr. Thorsten Peuker

      Article first published online: 8 JAN 2013 | DOI: 10.1002/cite.201200233

      No abstract.

    5. Flexible, Scalable and Configurable Single-Use Systems for Biopharmaceutical Research and Manufacture (pages 34–39)

      Detlev Szarafinski

      Article first published online: 7 NOV 2012 | DOI: 10.1002/cite.201200138

      Thumbnail image of graphical abstract

      While single-use components are state-of-the-art in the biopharmaceutical industry, there is still a need for systems that include several process steps. New scalable and configurable single-use systems, such as those for the production of monoclonal antibodies, are presented which incorporate unit operations like buffer and media preparation and cell cultivation.

  9. Reviews

    1. Top of page
    2. Titelbild
    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. Bioengineering Parameters for Single-Use Bioreactors: Overview and Evaluation of Suitable Methods (pages 40–56)

      Christian Löffelholz, Ute Husemann, Dr. Gerhard Greller, Dr. Wolfram Meusel, Jörg Kauling, Dr. Peter Ay, Dr. Matthias Kraume, Dr. Regine Eibl and Dr. Dieter Eibl

      Article first published online: 27 NOV 2012 | DOI: 10.1002/cite.201200125

      Thumbnail image of graphical abstract

      Well-known methods used for the characterization of conventional bioreactors are discussed and modified in order to characterize single-use bioreactors. If power consumption, the cultivation vessel's shape, its configuration and its scale are considered, a recommendation for preferred characterization methods for single-use bioreactors can finally be made.

    2. Cultivation of Cells and Microorganisms in Wave-Mixed Disposable Bag Bioreactors at Different Scales (pages 57–66)

      Dr. Stefan Junne, Thomas Solymosi, Dr. Nico Oosterhuis and Prof. Peter Neubauer

      Article first published online: 13 DEC 2012 | DOI: 10.1002/cite.201200149

      Thumbnail image of graphical abstract

      Wave-mixed bioreactors are characterized by low shear forces while gas exchange is realized through the large gas-liquid interface. This article describes applications of wave-mixed systems, current developments and as example the scale-up of an Escherichia coli fed-batch cultivation from the 12-L to 120-L scale in a disposable bioreactor.

    3. hMSC Production in Disposable Bioreactors with Regards to GMP and PAT (pages 67–75)

      Katharina Cierpka, Christiane L. Elseberg, Dr. Knut Niss, Prof. Dr. Moustapha Kassem, Dr.-Ing. Denise Salzig and Prof. Dr.-Ing. Peter Czermak

      Article first published online: 19 DEC 2012 | DOI: 10.1002/cite.201200151

      Thumbnail image of graphical abstract

      Human mesenchymal stem cells are of great interest for the use in regenerative medicine. The specialty of their production process, that the cell itself is the product, leads to completely different process requirements compared to existing cell production processes. To meet these requirements disposable reactor concepts are very promising.

    4. Functionalized Magnetizable Particles for Downstream Processing in Single-Use Systems (pages 76–86)

      Dr. Nils Tippkötter, Huschyar Al-Kaidy, Steffen Wollny and Prof. Dr. Roland Ulber

      Article first published online: 30 OCT 2012 | DOI: 10.1002/cite.201200130

      Thumbnail image of graphical abstract

      A significant reduction of unit operations in biotechnological downstream processing can be achieved by magnetic particles functionalized with affinity ligands. The principles of magnetizable particles, their synthesis and functionalization are explained and applications in the area of microfluidics and downstream processing are considered with a focus on single-use technology development potential.

  10. Research Articles

    1. Top of page
    2. Titelbild
    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. Fast Track API Manufacturing from Shake Flask to Production Scale Using a 1000-L Single-Use Facility (pages 87–94)

      Benjamin Minow, Hugo de Witt and Dr. Ingo Knabben

      Article first published online: 13 DEC 2012 | DOI: 10.1002/cite.201200136

      Thumbnail image of graphical abstract

      The necessity to shorten development timelines and efficiently manufacture biological drugs due to a highly increased competition on therapeutic targets, is forcing biotech companies to alter their production strategies. Single-use technologies in combination with platform processes and a pragmatic scale-up concept enable a significant reduction in timeline from lab to production.

    2. Fluid Flow and Cell Proliferation of Mesenchymal Adipose-Derived Stem Cells in Small-Scale, Stirred, Single-Use Bioreactors (pages 95–102)

      Stephan Kaiser, Valentin Jossen, Carmen Schirmaier, Prof. Dieter Eibl, Dr. Silke Brill, Christian van den Bos and Prof. Regine Eibl

      Article first published online: 19 DEC 2012 | DOI: 10.1002/cite.201200180

      Thumbnail image of graphical abstract

      The fluid flow and suspension characteristics in small-scale, stirred, single-use bioreactors were investigated experimentally and numerically. The required impeller speeds for homogenous suspension and the resulting shear stress levels were determined. The microcarrier type significantly influences the impeller speed required to achieve the desired suspension characteristics.

    3. Integrated Clinical Scale Manufacturing System for Cellular Products Derived by Magnetic Cell Separation, Centrifugation and Cell Culture (pages 103–110)

      Dr. Michael Apel, Dr. Mareke Brüning, Markus Granzin, Mike Essl, Juliane Stuth, Jessica Blaschke, Iris Spiegel, Sabine Müller, Eiad Kabaha, Elmar Fahrendorff, Stefan Miltenyi, Dr. Jürgen Schmitz, Dr. Dirk Balshüsemann and Volker Huppert

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201200175

      Thumbnail image of graphical abstract

      A new platform technology for cell processing is introduced that facilitates fully automated manufacturing of cellular products for clinical applications in a single-use tubing set. This functionally closed disposable integrates multiple workflows like cell washing, labeling, separation and cultivation. Thereby, it may allow advancing innovative research into next generation cellular therapies for routine use.

    4. Increase of Protein Yield in High Five Cells in a Single-Use Perfusion Bioreactor by Medium Replacement (pages 111–117)

      Dr.-Ing. Mareike Rausch, Prof. Dr.-Ing. Ralf Pörtner and Dr. Jörg Knäblein

      Article first published online: 21 NOV 2012 | DOI: 10.1002/cite.201200121

      Thumbnail image of graphical abstract

      For fast and cost-effective production of heterologous proteins the baculovirus expression vector system propagated on High-Five insect cells was used as an expression system. For this purpose a process was developed based on a single-use technology and a medium replacement strategy with cell retention.

    5. An Approach for Scale-Up of Geometrically Dissimilar Orbitally Shaken Single-Use Bioreactors (pages 118–126)

      Sören Werner, Johanna Olownia, Daniel Egger and Prof. Dr.-Ing. Dieter Eibl

      Article first published online: 19 NOV 2012 | DOI: 10.1002/cite.201200153

      Thumbnail image of graphical abstract

      Orbitally shaken single-use bags with square footprints offer a simple and cost-efficient alternative to present systems for scale-up processes and were characterized in regard to their engineering parameters and their potential for cultivating animal and plant suspension cell lines.

    6. Novel, Rotary Oscillated, Scalable Single-Use Bioreactor Technology for the Cultivation of Animal Cells (pages 127–135)

      Jörg Kauling, Dr. Helmut Brod, Dr. Marc Jenne, Annette Waldhelm, Dr. Uwe Langer and Dr. Berthold Bödeker

      Article first published online: 19 DEC 2012 | DOI: 10.1002/cite.201200155

      Thumbnail image of graphical abstract

      A novel single-use bioreactor is presented that utilizes the rotary oscillation of a polyhedral container as working principle. Thereby, also highly shear sensitive cell suspensions or microcarrier cultures can be cultivated in unmatched scales by employing simple, empty, robust and cost-efficient single-use 3D-bags.

    7. Development of the Travelling Wave Bioreactor – A Concept Study (pages 136–143)

      Stephan C. Kaiser, Prof. Matthias Kraume and Prof. Dieter Eibl

      Article first published online: 9 NOV 2012 | DOI: 10.1002/cite.201200127

      Thumbnail image of graphical abstract

      The concept of the orbitally shaken, traveling wave bioreactor is introduced. A numerical model was developed for the simulation of fluid flow in two different bioreactor geometries. The orbital motion combined with the toroidal shape of the bioreactor create the desired wave characteristics in the contents of the vessel.

    8. Investigations on Mechanical Stress Caused to CHO Suspension Cells by Standard and Single-Use Pumps (pages 144–152)

      Katharina Blaschczok, Stephan C. Kaiser, Christian Löffelholz, Nicole Imseng, Jürg Burkart, Dr. Pascal Bösch, Wolfgang Dornfeld, Prof. Regine Eibl and Prof. Dieter Eibl

      Article first published online: 19 NOV 2012 | DOI: 10.1002/cite.201200135

      Thumbnail image of graphical abstract

      Mechanical stress exerted on transfected CHO suspension cells by reusable and single-use magnetically levitated centrifugal-type pumps was investigated. Cell densities and viabilities were compared with data from a peristaltic and a 4-piston diaphragm pump. Considerably lower cell death rates were detected when using the centrifugal-type pumps.

    9. Bioprocess Development in Single-Use Systems for Heterotrophic Marine Microalgae (pages 153–161)

      Friederike Hillig, Steffi Annemüller, Magdalena Chmielewska, Dr.-Ing. Maciej Pilarek, Dr.-Ing. Stefan Junne and Prof. Dr. Peter Neubauer

      Article first published online: 9 NOV 2012 | DOI: 10.1002/cite.201200143

      Thumbnail image of graphical abstract

      Application of orbital shaken polymer based systems for the process development in three stages for marine shear-sensitive microorganism is demonstrated. Experiments including experimental design approaches show the influence of hydrodynamic forces on growth and cell physiology and underline the importance of the choice of the matching cultivation system.

    10. High Cell Density Escherichia coli Cultivation in Different Single-Use Bioreactor Systems (pages 162–171)

      Thomas Dreher, Ute Husemann, Christian Zahnow, Davy de Wilde, Dr. Thorsten Adams and Dr. Gerhard Greller

      Article first published online: 2 NOV 2012 | DOI: 10.1002/cite.201200122

      Thumbnail image of graphical abstract

      Single-use bioreactors are established for cell culture processes and offer also advantages for microbial fermentations. Due to limitations in the oxygen transfer rate reports of microbial fermentations are rarely found. This study shows high cell density Escherichia coli fermentations in different single-use bioreactors, where the achieved cell densities are comparable to processes in stainless steel fermenters.

    11. Pre-Calibrated Biosensors for Single-Use Applications (pages 172–178)

      Dr. Isabella Moser and Gerhard Jobst

      Article first published online: 19 DEC 2012 | DOI: 10.1002/cite.201200129

      Thumbnail image of graphical abstract

      A generic technology platform, based on polymer laminate technologies with integrated electrochemical biosensors utilizing nanocomposite membranes, allows the fabrication of fully flexible devices comprising biosensor arrays for lactate, glucose, glutamine, and glutamate. The biosensors are pre-calibrated and assembled to 0.15-μL flow-through cells on wafer level.

    12. Application of High Frequency Sensors for Contactless Monitoring in Disposable Bioreactors (pages 179–185)

      Thomas Nacke, Andreas Barthel, Dr. Dieter Frense, Matthias Meister and Dr. Brian P. Cahill

      Article first published online: 27 NOV 2012 | DOI: 10.1002/cite.201200147

      Thumbnail image of graphical abstract

      A contamination-free process measurement by means of high-frequency sensors for the contactless measurement of the permittivity and conductivity of fermentation media is presented. Microwaves can penetrate through plastic materials with low attenuation enabling contactless sensing of cell growth in single-use bioreactors with long-term stability.

    13. Determination of Extractables and Leachables from Single-Use Systems (pages 186–196)

      Weibing Ding

      Article first published online: 15 NOV 2012 | DOI: 10.1002/cite.201200113

      Thumbnail image of graphical abstract

      Although single-use systems are commonly used in biopharmaceutical production the issue of extractables and leachables that possibly migrate to the products is unsolved. Therefore, a practical, science-based approach for determination of extractables and leachables from single-use systems is presented on the example of a case study.

  11. Communications

    1. Top of page
    2. Titelbild
    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. Wave-Mixed and Orbitally Shaken Single-Use Photobioreactors for Diatom Algae Propagation (pages 197–201)

      Nicolai Lehmann, Dr. Heiko Rischer, Prof. Dr. Dieter Eibl and Prof. Dr. Regine Eibl

      Article first published online: 3 DEC 2012 | DOI: 10.1002/cite.201200137

      Thumbnail image of graphical abstract

      Bubble-induced hydrodynamic stress can cause cell damage in cultivation of diatoms. Therefore, the growth of the model microalgae Phaeodactylum tricornutum was investigated using three different single-use bioreactors suitable for cultivation of shear-sensitive cells. The highest cell density was obtained in orbitally shaken, 2D bag equipped with cool-white fluorescent tubes.

    2. Disposable Flat Panel Airlift Photobioreactors (pages 202–205)

      Peter Bergmann, Dr. Peter Ripplinger, Lars Beyer and Prof. Dr. Walter Trösch

      Article first published online: 28 NOV 2012 | DOI: 10.1002/cite.201200132

      Thumbnail image of graphical abstract

      Single-use bioreactors are increasingly applied in biotechnological processes due to their high flexibility and easy handling, as well as the minimized risk of cross-contamination. An innovative flat panel airlift photobioreactor is introduced for the production of microalgae.

  12. Buchbesprechungen

    1. Top of page
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    4. Editorial
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    6. Aus den Gesellschaften
    7. Personalien
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    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. Drying in the Process Industry. Von C. M. van't Land. (pages 206–207)

      T. Metzger

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390005

    2. Industrial Biofouling. Von T. R. Bott. (page 207)

      W. Sand

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390006

  13. Vorschau

    1. Top of page
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    4. Editorial
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    6. Aus den Gesellschaften
    7. Personalien
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    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. Vorschau: Chem. Ing. Tech. 1-2/2013 (page 210)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390007

  14. Überblick

    1. Top of page
    2. Titelbild
    3. Innentitelbild
    4. Editorial
    5. Inhalt
    6. Aus den Gesellschaften
    7. Personalien
    8. Forum
    9. Essays
    10. Reviews
    11. Research Articles
    12. Communications
    13. Buchbesprechungen
    14. Vorschau
    15. Überblick
    1. You have free access to this content
      Überblick Inhalt: Chem. Eng. Technol. 2/2013 (page 211)

      Article first published online: 25 JAN 2013 | DOI: 10.1002/cite.201390008

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