CO2 sequestration using principles of shell formation

Authors

  • Seung-Woo Lee,

    Corresponding author
    1. CO2 Sequestration Research Department, Korea Institute of Geoscience and Mineral Resources, Gwahangno 92, Yuseonggu, Daejeon 305-350, Korea
    • CO2 Sequestration Research Department, Korea Institute of Geoscience and Mineral Resources, Gwahangno 92, Yuseonggu, Daejeon 305-350, Korea.
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  • Si-Hyun Lee,

    1. Energy Conversion Research Department of Clean Energy System Research Center, Korea Institute of Energy Research, Jangdong, Yuseonggu, Daejeon 305-343, Korea
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  • Young-Nam Jang,

    1. CO2 Sequestration Research Department, Korea Institute of Geoscience and Mineral Resources, Gwahangno 92, Yuseonggu, Daejeon 305-350, Korea
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  • Kyoung-Soo Lim,

    1. Energy Conversion Research Department of Clean Energy System Research Center, Korea Institute of Energy Research, Jangdong, Yuseonggu, Daejeon 305-343, Korea
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  • Soon-Kwan Jeong

    Corresponding author
    1. Energy Conversion Research Department of Clean Energy System Research Center, Korea Institute of Energy Research, Jangdong, Yuseonggu, Daejeon 305-343, Korea
    • Energy Conversion Research Department of Clean Energy System Research Center, Korea Institute of Energy Research, Jangdong, Yuseonggu, Daejeon 305-343, Korea.
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  • This study was not done with research on human subjects or experimental animals.

Abstract

Biomimetic sequestration of carbon dioxide (CO2) is one of the methods proposed to reduce the CO2 released into the atmosphere. In this study, we compared soluble protein of hemocyte from diseased shell (HDS) and extrapallial fluid (EPF) extracted from Crassostrea gigas with bovine carbonic anhydrase II in terms of their ability to promote CO2 hydration and the production of calcium precipitates. On the basis of the experiments of CO2 hydration, the key role of HDS was identified. Moreover, mass-spectroscopic analysis (MALDI-TOF) and circular dichroism (CD) analysis were used for understanding molecular weight and secondary protein structure. From the amino acid sequence and secondary protein structure, the different processes of CO2 hydration by bovine carbonic anhydrase II and HDS could be assessed.

Abstract

La séquestration biomimétique du dioxyde de carbone (CO2) est l'une des méthodes proposées pour réduire la quantité de CO2 rejetée dans l'atmosphère. Dans cette étude, nous avons comparé la protéine soluble de l'hémocyte de coquillages malades (HCM) et le fluide extra-palléal (FEP) extrait de Crassostrea gigas avec l'anhydrase carbonique bovine II. La comparaison a porté sur leur capacité à favoriser l'hydratation du CO2 et la production de précipités de calcium. Grâce aux expériences sur l'hydratation du CO2, le rôle primordial du HCM a été déterminé. Par ailleurs, la spectroscopie de masse (MALDI-TOF) et l'analyse du dichroïsme circulaire (DC) ont été utilisées pour déterminer la masse moléculaire et la structure secondaire protéique. La séquence d'acides aminés et la structure secondaire protéique ont permis d'évaluer les différents procédés d'hydratation du CO2 au moyen de l'anhydrase carbonique bovine II et du HCM. © 2011 Canadian Society for Chemical Engineering

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