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Non-selective hydrolysis of tuna fish oil for producing free fatty acids containing docosahexaenoic acid

Authors

  • Aditi Sharma,

    1. Chemical Engineering Department, Banasthali University, Jaipur, Rajasthan, India
    2. Department of Chemical and Biological Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada
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  • Satyendra P. Chaurasia,

    1. Chemical Engineering Department, Malaviya National Institute of Technology, Jaipur, Rajasthan, India
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  • Ajay K. Dalai

    Corresponding author
    1. Department of Chemical and Biological Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada
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  •  •Immobilised Candida antarctica lipase B (CAL-B) and support material (immobead-150) were characterised.
  •  •The Michaelis–Menten kinetic model and activation energy were studied for hydrolysis.
  •  •Activity of CAL-B was studied up to five runs of repeated use.
  •  •Tuna fish oil was effectively hydrolysed with CAL-B to produce DHA-rich free fatty acids.

Abstract

The nature of immobilised Candida antarctica lipase-B (CAL-B) catalysed hydrolysis of tuna fish oil was studied with parameters such as solvent and water concentration, temperature, speed of agitation and enzyme loading. Immobilised CAL-B and support material immobead-150 were characterised with BET surface area, particle size analyser and Fourier transform infrared spectroscopy (FT-IR) to record their physiochemical properties. The maximum rate of reaction (Vmax) of 500 µmol of free fatty acids (FFAs) per mL reaction mixture per h and Michaelis−Menten constant (KM) of 2115 µmol FFAs/mL were found for Michaelis−Menten type kinetic model. Activation energy (E) of 26.1 KJ/mol was calculated for immobilised CAL-B. The 55.9% conversion of triglycerides was observed after the third use of the immobilised CAL-B. The activity retention of immobilised CAL-B reduced to 33.5% after the fourth repeated use of the enzyme.

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