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Full Paper
Spectrofluorimetric Determination and Validation of Biotin in Pure and Dosage Form via Derivatization with 4-Fluoro-7-nitrobenzofurazan
Article first published online: 30 NOV 2010
DOI: 10.1002/cjoc.201090365
Copyright © 2010 SIOC, CAS, Shanghai & WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
1614-7065/asset/cover.gif?v=1&s=d8f82c270f66bd5689c2415620742b3baee364c2 "Chinese Journal of Chemistry")
Additional Information
How to Cite
Sevgi, T. U. (2010), Spectrofluorimetric Determination and Validation of Biotin in Pure and Dosage Form via Derivatization with 4-Fluoro-7-nitrobenzofurazan. Chin. J. Chem., 28: 2209–2215. doi: 10.1002/cjoc.201090365
Publication History
- Issue published online: 30 NOV 2010
- Article first published online: 30 NOV 2010
- Manuscript Accepted: 21 JUL 2010
- Manuscript Received: 30 DEC 2009
Funded by
- the Research Fund of University of ?stanbul. Grant Number: BYP-8362
- Abstract
- References
- Cited By
Keywords:
- biotin;
- 4-fluoro-7-nitrobenzofurazan;
- derivatization;
- validation
Abstract
A simple and highly sensitive spectrofluorimetric method was developed for the determination of biotin in pure and dosage form. The method is based on the derivatization of biotin with 4-fluoro-7-nitrobenzofurazan in borate buffer of pH 9.0 to yield a yellow, fluorescent product. The various chemical conditions that affected the reaction were studied. The method was validated for specificity, linearity, precision, accuracy, recovery and robustness. At optimized experimental conditions, a linear relationship between the fluorescence intensity of the concentration of biotin is observed in the range 45–450 ng/mL. Limit of detection and quantification were 0.038 and 0.114 ng/mL, respectively. The percentage mean recovery was 99.96. The proposed procedure was successfully applied to the determination of biotin in its dosage form with mean recovery of 101.23±1.22 for biotin tablets. The results obtained were in good agreement with those obtained by the reference method.