Orthogonal Enzymatic Reactions to Control Supramolecular Hydrogelations

Authors

  • Guoqin Chen,

    1. Department of Cardiology, Guangzhou Panyu Central Hospital, Guangzhou, Guangdong 511400, China
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  • Chunhua Ren,

    1. College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300071, China
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  • Ling Wang,

    1. College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, 300071, China
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  • Bing Xu,

    Corresponding author
    1. Department of Chemistry, The Hong Kong University of Science and Technology, Hongkong, China
    2. Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02468, USA
    • Bing Xu, Department of Chemistry, The Hong Kong University of Science and Technology, Hongkong, China

      Zhimou Yang, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China, Tel.: 0086-022-23502875; Fax: 0086-022-23498775

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  • Zhimou Yang

    Corresponding author
    1. Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China
    2. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, China
    • Bing Xu, Department of Chemistry, The Hong Kong University of Science and Technology, Hongkong, China

      Zhimou Yang, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China, Tel.: 0086-022-23502875; Fax: 0086-022-23498775

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Abstract

Enzyme-responsive hydrogels have great potential in applications of controlled drug release, tissue engineering, etc. In this study, we reported on a supramolecular hydrogel that showed responses to two enzymes, phosphatase which was used to form the hydrogels and esterase which could trigger gel-sol phase transitions. The gelation process and visco-elasticity property of the resulting gel, morphology of the nanostructures in hydrogel, and peptide conformation in the self-assembled nanostructure were characterized by rheology, transmission electron microscope (TEM), and circular dichroism (CD), respectively. Potential application of the enzyme-responsive hydrogel in drug release was also demonstrated in this study. Though only one potential application of drug release was proved in this study, the responsive hydrogel system in this study might have potentials for the applications in fields of cell culture, controlled-drug release, etc.

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