Facile Fabrication of a Graphene-based Electrochemical Biosensor for Glucose Detection

Authors

  • Yanqin Zhang,

    1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
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  • Youjun Fan,

    Corresponding author
    1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
    • Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
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  • Shanshan Wang,

    1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
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  • Yiliang Tan,

    1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
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  • Xingcan Shen,

    Corresponding author
    1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
    • Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China
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  • Zujin Shi

    1. Beijing National Laboratory for Molecular Sciences, State Key Lab of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
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Abstract

A simple and effective glucose biosensor based on immobilization of glucose oxidase (GOD) in graphene (GR)/Nafion film was constructed. The results indicated that the immobilized GOD can maintain its native structure and bioactivity, and the GR/Nafion film provides a favorable microenvironment for GOD immobilization and promotes the direct electron transfer between the electrode substrate and the redox center of GOD. The electrode reaction of the immobilized GOD shows a reversible and surface-controlled process with the large electron transfer rate constant (ks) of 3.42±0.08 s−1. Based on the oxygen consumption during the oxidation process of glucose catalyzed by the immobilized GOD, the as-prepared GOD/GR/Nafion/GCE electrode exhibits a linear range from 0.5 to 14 mmol·L−1 with a detection limit of 0.03 mmol·L−1. Moreover, it displays a good reproducibility and long-term stability.

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