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Keywords:

  • polyelectrolyte;
  • dynamic light scattering;
  • fluorescence correlation spectroscopy;
  • hydrodynamic radius

Abstract

The advantage of fluorescence correlation spectroscopy to study single chain behavior of polyelectrolytes has been demonstrated by checking the coil-to-globule transition of poly 2-vinylpyridine with the change of pH value in aqueous solution. The ultra-high sensitivity of FCS allows measurement at extreme dilution where the effect of electrostatic interaction between the chains is greatly suppressed. The results exposed first-order conformation transition of P2VP as detected by FCS while inter-chain aggregation occurred in the experiments of dynamic light scattering.