Advantage of Fluorescence Correlation Spectroscopy for the Study of Polyelectrolytes

Authors

  • Pengxiang Jia,

    1. Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710069, China
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  • Yongkuan Gong,

    1. Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry and Materials Science, Northwest University, Xi'an, Shaanxi 710069, China
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  • Shengqin Wang,

    1. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
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  • Jiang Zhao

    Corresponding author
    1. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China
    • Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China, Tel.: 0086-10-82619847; Fax: 0086-10-82619847
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Abstract

The advantage of fluorescence correlation spectroscopy to study single chain behavior of polyelectrolytes has been demonstrated by checking the coil-to-globule transition of poly 2-vinylpyridine with the change of pH value in aqueous solution. The ultra-high sensitivity of FCS allows measurement at extreme dilution where the effect of electrostatic interaction between the chains is greatly suppressed. The results exposed first-order conformation transition of P2VP as detected by FCS while inter-chain aggregation occurred in the experiments of dynamic light scattering.

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