Exploring Quaternized Hydroxyethylcellulose as Potential Gene Carriers

Authors

  • Fei Ding,

    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China
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  • Jun You,

    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China
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  • Xiaocheng Weng,

    Corresponding author
    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China
    • College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China, Tel.: 0086-027-68756663
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  • Jinping Zhou,

    Corresponding author
    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China
    • College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China, Tel.: 0086-027-68756663
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  • Xiaolian Zhang,

    1. School of Medicine, Wuhan University, Hubei, Wuhani 430071, China
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  • Xiang Zhou,

    Corresponding author
    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China
    2. State Key Lab of Bioorganic & Natural Product Chemistry, Shanghai Institute of Organic Chememistry, Shanghai 200032, China
    • College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China, Tel.: 0086-027-68756663
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  • Lina Zhang

    1. College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China
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Abstract

Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose (QHEC) derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and resist the degradation of DNase I. And the dynamic light scatter (DLS) results showed that all QHEC/DNA complexes could form compact particles. These QHEC/DNA complexes exhibited effective transfection abilities in comparison to the naked DNA. The cytotoxicities of QHEC and QHEC/DNA complexes were also evaluated in four cell lines which were relatively low compared with 25 kDa bPEI. All results indicated that these quaternized hydroxyethylcelluloses could be used as potential gene delivery vectors.

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