The rod-like oligo(p-phenylene ethynylene)-functionalized perylene bisimide triad was synthesized and characterized. Aggregation behavior in solvents of different polarity was investigated by absorption and fluorescent spectroscopy. The results showed that stronger aggregations took place in low-polarity slovent. The experiments also indicated that the energy and electron transfer might takeplace between the two chromophores during the photoinduced excitation. Highly ordered two-dimensional assemblies could be observed at solid/liquid interfaces.